Isomerization of linolenic acid by rumen micro-organisms

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The biohydrogenation of alpha-linolenic acid and oleic acid by rumen micro-organisms.

1. alpha-[U-(14)C]Linolenic acid was incubated with the rumen contents of sheep and the metabolic products were characterized by thin-layer chromatography, gas-liquid chromatography and absorption spectroscopy in the ultraviolet and infrared. 2. A tentative scheme for the biohydrogenation route to stearic acid is presented. The main pathway is through diconjugated cis-cis-cis-octadecatrienoic a...

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Proteins and peptides were acetylated using acetic anhydride in order to block their N-terminal amino groups and thereby to prevent their hydrolysis by rumen microbial aminopeptidases. The effects of acetylation on peptide breakdown and ammonia production were determined by incubating unmodified and acetylated substrates with sheep rumen micro-organisms in vitro. Ammonia production from casein ...

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Acetylation of peptides inhibits their degradation by rumen micro-organisms

Proteins and peptides were acetylated using acetic anhydride in order to block their N-terminal amino groups and thereby to prevent their hydrolysis by rumen microbial aminopeptidases. The effects of acetylation on peptide breakdown and ammonia production were determined by incubating unmodified and acetylated substrates with sheep rumen micro-organisms in vitro. Ammonia production from casein ...

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Avery, 0. T. & Goebel, W. F. (1929). J. exp. Med. 50, 533. Bates, F. J. (1942). Polarimetry, Saccharimetry and the Sugare, pp. 493, 494. Washington, D.C.: United States Government Printing Office. Beiser, S. M., Burke, G. C. & Tanenbaum, S. W. (1960). J. molec. Biol. 2, 125. Fischer, E. (1910). Ber. dtsch. chem. Ge8. 48, 2534. Goebel, W. F., Avery, 0. T. & Babers, F. H. (1934). J. exp. Med. 60,...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1968

ISSN: 0306-3283

DOI: 10.1042/bj1090477